Objective To construct recombinant cosmid vector pAxCAwt MC148 from the MC148 gene of molluscum contangiosum virus(MCV).
目的构建传染性软疣病毒(MCV )MC14 8基因腺病毒重组粘粒。
METHODS: Angiopoietin-1-PAxCAwt cosmid DNA and adenoviral DNA-TPC were cotransfected to 293 cells by the use of calcium phosphate precipitation method.